Electric-field and photochemical effects in D85N mutant bacteriorhodopsin substituted with 4-keto-retinal
| Autor: | Evgeniy P. Lukashev, Anna B. Druzhko, Yuan-chin Ching, Paul Kolodner |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Schiff base
genetic structures biology Metals and Alloys Bacteriorhodopsin Protonation Retinal Surfaces and Interfaces Photochemistry Surfaces Coatings and Films Electronic Optical and Magnetic Materials Pigment chemistry.chemical_compound Deprotonation chemistry Electrochromism visual_art Materials Chemistry visual_art.visual_art_medium biology.protein sense organs Absorption (chemistry) |
| Zdroj: | Thin Solid Films. 302:231-234 |
| ISSN: | 0040-6090 |
| Popis: | All- trans 4-keto-retinal has been incorporated into bleached apomembranes of D85N mutant bacteriorhodopsin. The resulting pigment exhibits a pH-dependent equilibrium between forms with a protonated (pink) and an unprotonated (yellow) Schiff base, with pK −6.15. Optical absorption changes produced by flash illumination of suspensions of the protonated and unprotonated forms have been recorded. Applying an electric field across a thin, dry film of the pink form of the pigment produces spectral changes characteristic of Schiff-base deprotonation. These experiments demonstrate that electric-field induced Schiff-base deprotonation. First discovered in blue D85N bacteriorhodopsin, can be the basis of electrochromic materials of different colors made using retinal—analog substitution as well as genetic mutation. |
| Databáze: | OpenAIRE |
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