Effect of Nitro Orientation on Ras -Protooncogene Mutation in Liver Tumors from 7-Nitrodibenz[ a,h ]anthracene-Treated Mice
| Autor: | De-Jin Zhan, Qingsu Xia, Robert H. Heflich, Peter P. Fu, Linda S. Von Tungeln |
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| Rok vydání: | 2002 |
| Předmět: | |
| Zdroj: | Polycyclic Aromatic Compounds. 22:853-859 |
| ISSN: | 1563-5333 1040-6638 |
| DOI: | 10.1080/10406630290104004 |
| Popis: | Dibenz[ a,h ]anthracene (DB[ a,h ]A) and 7-nitrodibenz[ a,h ]anthracene (7-NDB[ a,h ]A) induced liver tumors when administered to neonatal B6C3F 1 mice. For protooncogene analysis, RNA was isolated from each of the liver tumors from treated mice and reverse-transcribed into cDNA. Portions of the K- and H- ras protein coding sequences were then amplified and analyzed for DNA sequence alterations. DB[ a,h ]A-induced liver tumors had a 100% (23/23) frequency of ras -protooncogene mutation, with 83% (19/23) occurring at the first base of K- ras codon 13 and resulting in G GC M C GC transversion; the remaining 17% (4/23) of the mutations were located at the second base of H- ras codon 61. In contrast, only four of nine (44%) of 7-NDB[ a,h ]A-induced liver tumors had ras -protooncogene mutations, with two each at K- ras codon 13 and H- ras codon 61. Combined with previous observations, the results indicate that the nitro substituent perpendicular to the aromatic moiety alters the chemical-induced protooncogene ... |
| Databáze: | OpenAIRE |
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