Isolation of Rat and Mouse Alveolar Type II Epithelial Cells
| Autor: | Juan R. Alvarez, Jazalle McClendon, Zea Borok, Hidenori Kage, Mitsuhiro Sunohara, Nicole L. Jansing, Rachel L. Zemans |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Lung Chemistry Alveolar Epithelium respiratory system Immunomagnetic separation Epithelium Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure 030228 respiratory system Pulmonary surfactant medicine Progenitor cell Ion transporter Function (biology) |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493985692 |
| DOI: | 10.1007/978-1-4939-8570-8_6 |
| Popis: | The gas exchange surface of the lungs is lined by an epithelium consisting of alveolar type (AT) I and ATII cells. ATII cells function to produce surfactant, play a role in host defense and fluid and ion transport, and serve as progenitors. ATI cells are important for gas exchange and fluid and ion transport. Our understanding of the biology of these cells depends on the investigation of isolated cells. Here, we present methods for the isolation of mouse and rat ATII cells. |
| Databáze: | OpenAIRE |
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