Optimization of submerged culture conditions involving a developed fine powder solid seed for exopolysaccharide production by the medicinal mushroom Ganoderma lucidum
| Autor: | Wei-Rui Zhang, Sheng-Rong Liu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Chemistry Soybean meal food and beverages Biomass 04 agricultural and veterinary sciences 040401 food science 01 natural sciences Applied Microbiology and Biotechnology 0404 agricultural biotechnology Medicinal mushroom Fine powder 010608 biotechnology Food science Response surface methodology Water content Mycelium Food Science Biotechnology Ganoderma lucidum |
| Zdroj: | Food Science and Biotechnology. 28:1135-1145 |
| ISSN: | 2092-6456 1226-7708 |
| DOI: | 10.1007/s10068-018-0536-5 |
| Popis: | To facilitate Ganoderma lucidum submerged cultivation and achieve high productivity, four fine powder solid substrates incorporated with different nitrogen-rich supplements were utilized to grow the fungus and as solid seed for its submerged culture. Of the four solid seeds, the soybean meal solid seed gave the highest biomass (10.73 g/L) and exopolysaccharide (EPS) (1.22 g/L), higher than those (8.36 g/L biomass and 0.44 g/L EPS) obtained with mycelial liquid seed. The optimal level of soybean meal supplementation was 20% (w/w) for production of the solid seed. Following single factor experiments, levels of three selected process variables were optimized as: the moisture content of solid seed, 70%; inoculum size, 0.8 g/flask; and rotary speed, 160 rpm. These conditions were validated experimentally with improved EPS yield of 1.33 g/L. The developed solid seed can be conveniently used for G. lucidum submerged culture with improved EPS productivity. |
| Databáze: | OpenAIRE |
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