Role of IFNLR1 gene in PRRSV infection of PAM cells
| Autor: | Lixia Ma, Jing Zhao, Wei Chen, Yongqing Zeng, Ming Qin, Yu Zhang, Zhi-Xin Li, Jinhong Geng, Lixue Wang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Gene knockdown General Veterinary biology 040301 veterinary sciences JAK-STAT signaling pathway 04 agricultural and veterinary sciences Porcine reproductive and respiratory syndrome virus biology.organism_classification Virus 0403 veterinary science 03 medical and health sciences Immune system Downregulation and upregulation Cancer research Signal transduction Cytokine receptor 030304 developmental biology |
| Zdroj: | Journal of Veterinary Science. 22 |
| ISSN: | 1976-555X 1229-845X |
| DOI: | 10.4142/jvs.2021.22.e39 |
| Popis: | Background Interferon lambda receptor 1 (IFNLR1) is a type II cytokine receptor that clings to interleukins IL-28A, IL29B, and IL-29 referred to as type III IFNs (IFN-λs). IFN-λs act through the JAK-STAT signaling pathway to exert antiviral effects related to preventing and curing an infection. Although the immune function of IFN-λs in virus invasion has been described, the molecular mechanism of IFNLR1 in that process is unclear. Objectives The purpose of this study was to elucidate the role of IFNLR1 in the pathogenesis and treatment of porcine reproductive and respiratory syndrome virus (PRRSV). Methods The effects of IFNLR1 on the proliferation of porcine alveolar macrophages (PAMs) during PRRSV infection were investigated using interference and overexpression methods. Results In this study, the expressions of the IFNLR1 gene in the liver, large intestine, small intestine, kidney, and lung tissues of Dapulian pigs were significantly higher than those in Landrace pigs. It was determined that porcine IFNLR1 overexpression suppresses PRRSV replication. The qRT-PCR results revealed that overexpression of IFNLR1 upregulated antiviral and IFN-stimulated genes. IFNLR1 overexpression inhibits the proliferation of PAMs and upregulation of p-STAT1. By contrast, knockdown of IFNLR1 expression promotes PAMs proliferation. The G0/G1 phase proportion in IFNLR1-overexpressing cells increased, and the opposite change was observed in IFNLR1-underexpressing cells. After inhibition of the JAK/STAT signaling pathway, the G2/M phase proportion in the IFNLR1-overexpressing cells showed a significant increasing trend. In conclusion, overexpression of IFNLR1 induces activation of the JAK/STAT pathway, thereby inhibiting the proliferation of PAMs infected with PRRSV. Conclusion Expression of the IFNLR1 gene has an important regulatory role in PRRSV-infected PAMs, indicating it has potential as a molecular target in developing a new strategy for the treatment of PRRSV. |
| Databáze: | OpenAIRE |
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