Expression of human immunoglobulin E ε chain cDNA inE.coli
| Autor: | Tasuku Honjo, Koichi Igarashi, Yukio Sugino, Masakazu Kikuchi, Reiko Sasada, Haruo Onda, Tsutornu Kurokawa, Masaharu Seno, Yoshitaka Ona |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Physics::Biological Physics
Quantitative Biology::Biomolecules Antigenicity Molecular mass Biology medicine.disease_cause Quantitative Biology::Genomics Molecular biology Quantitative Biology::Subcellular Processes Immunoglobulin epsilon-Chains Plasmid Complementary DNA Genetics medicine Immunoglobulin heavy chain Escherichia coli Peptide sequence |
| Zdroj: | Nucleic Acids Research. 11:3077-3085 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/11.10.3077 |
| Popis: | Using the cDNA of human epsilon chain, three expression plasmids that code directly the constant portion of the epsilon chain (C epsilon 1-C epsilon 4, C epsilon 2-C epsilon 4 and C epsilon 3-C epsilon 4 domains) were constructed. These epsilon chain peptides were synthesized in E. coli under the control of the trp promoter-operator. The bacterially produced peptides have the antigenicity of human epsilon chain and gave the molecular weights equal to the values calculated from the amino acid sequence of the constructed plasmids. |
| Databáze: | OpenAIRE |
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