Boronate-functionalized hydrogel as a novel biosensing interface for the glycated hemoglobin A1c (HbA1c) based on the competitive binding with signaling glycoprotein
| Autor: | Yong Ju Yang, Kangsun Lee, Seung Yeon Song, Hyun C. Yoon, Ka Ram Kim, Yoo Min Park, Yong Duk Han, Yunhee Ku |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Materials science
endocrine system diseases Bioengineering 02 engineering and technology 01 natural sciences Horseradish peroxidase Biomaterials chemistry.chemical_compound Phenylboronic acid chemistry.chemical_classification Chromatography biology Chromogenic 010401 analytical chemistry nutritional and metabolic diseases 021001 nanoscience & nanotechnology 0104 chemical sciences Membrane chemistry Mechanics of Materials biology.protein Glycated hemoglobin 0210 nano-technology Glycoprotein Biosensor Boronic acid |
| Zdroj: | Materials Science and Engineering: C. 77:1160-1169 |
| ISSN: | 0928-4931 |
| DOI: | 10.1016/j.msec.2017.04.043 |
| Popis: | According to recent increases in public healthcare costs associated with diabetes mellitus, the development of new glycemic monitoring techniques based on the biosensing of glycated hemoglobin A1c (HbA 1c ), a promising long-term glycemic biomarker, has become a major challenge. In the development of HbA 1c biosensors for point-of-care applications, the selection of an effective biorecognition layer that provides a high reaction yield and specificity toward HbA 1c is regarded as the most significant issue. To address this, we developed a novel HbA 1c biosensing interfacial material by the integration of boronate hydrogel with glass fiber membrane. In the present study, a new boronate-functionalized hydrogel was designed and spatio-selectively photopolymerized on a hydrophilic glass fiber membrane by using N-hydroxyethyl acrylamide, 3-(acrylamido)phenylboronic acid, and bis( N , N ′-methylene-bis-acrylamide). Using this approach, the boronic acid group, which specifically recognizes the cis-diol residue of glucose on the HbA 1c molecule, can be three-dimensionally coated on the surface of the glass fiber network with a high density. Because this network structure of boronate hydrogel-grafted fibers enables capillary-driven fluid control, facile HbA 1c biosensing in a lateral flow assay concept could be accomplished. On the proposed HbA 1c biosensing interface, various concentrations of HbA 1c (5–15%) in blood-originated samples were sensitively measured by a colorimetric assay using horseradish peroxidase, a glycoenzyme can generate chromogenic signal after the competitive binding against HbA 1c to the boronic acid residues. Based on the demonstrated advantages of boronate hydrogel-modified membrane including high analytical performance, easy operation, and cost-effectiveness, we expect that the proposed biorecognition interfacial material can be applied not only to point-of-care HbA 1c biosensors, but also to the quantitative analysis of other glycoprotein biomarkers. |
| Databáze: | OpenAIRE |
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