Alpha-fetoprotein synthesis by clonogenic cells of rat hepatoma McA-RH 7777
| Autor: | G. I. Abelev, T. L. Éraizer |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
biology
Immunoperoxidase Red Cell Chemistry digestive oral and skin physiology Cell General Medicine medicine.disease Molecular biology digestive system diseases General Biochemistry Genetics and Molecular Biology Hemolysis medicine.anatomical_structure embryonic structures medicine biology.protein Antibody Clonogenic assay Alpha-fetoprotein Protein A neoplasms |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 94:1423-1426 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/bf00827219 |
| Popis: | Production of alpha-fetoprotein (AFP) was determined in single cells of hepatoma McA-RH7777 and in the clones of their progeny. To elucidate the heritability of this trait in a series of cell generations, a variety of local hemolysis in gel was devised. According to the method the cells and red cells conjugated with protein A were placed on the polylysine covered surface and layered with agarose gel containing antibodies. AFP production by single cells was determined from the formation of plaques--areas of red cell hemolysis. The cells forming the plaques (+AFP) and not forming them (-AFP) were distinguished and their reproduction was followed up. After 7-14 days the cells were fixed and stained by the immunoperoxidase technique with antibodies to AFP. High efficacy of the cloning has been demonstrated for both +AFP- and -AFP-cells (69 and 71%). Negative cells preserved their phenotype more frequently, producing homogenous negative clones, whereas +AFP cells gave "negative" clones in 1/3 of the cases. Both cells gave mixed clones in a small percentage of the cases. At present the AFP trait in these cells is being studied by recloning. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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