Comparison of the effect of L-lysine-?-oxidase fromTrichodern, harzianum Rifai andTrichoderma viride on nucleic acid synthesis in human tumor cellsin vitro

Autor:	Dobrynin IaV, Zhukova Os, Khaduev SKh, Soda K, Berezov Tt
Rok vydání:	1986
Předmět:	DNA synthesis biology Trichoderma viride RNA Trichoderma harzianum General Medicine biology.organism_classification Molecular biology General Biochemistry Genetics and Molecular Biology Enzyme assay chemistry.chemical_compound Biochemistry chemistry Trichoderma Nucleic acid biology.protein DNA
Zdroj:	Bulletin of Experimental Biology and Medicine. 101:666-667
ISSN:	1573-8221 0007-4888
DOI:	10.1007/bf00835895
Popis:	This paper gives comparative data on the effect of the new antitumor enzyme LO from a Soviet strain Trichoderma harzianum Rifai and from Trichoderma viride from Japan, on DNA and RNA synthesis in human ovarian carcinoma cells (CaOv strain) in culture, and also the results of the action of LO from T. harzianum Rifai on protein synthesis. Specific precursors were added before the end of the incubation time to the samples; /sup 3/H-thymidine as precursor for DNA synthesis, /sup 3/H-uridine as RNA precursor, and /sup 3/H-leucine as protein precursor. The final values of inhibition of DNA and RNA synthesis in the presence of enzyme from both sources are shown to depend in a virtually linear manner on enzyme concentration.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ec166a760f6dbc36b39e304b0a6054d9 https://doi.org/10.1007/bf00835895 Zobrazit plný text záznamu Full text from SpringerLink