Kit with technetium-99m labelled antimicrobial peptide UBI 29-41 for specific infection detection

Autor: Agnieszka Korsak, Hans I.J. Feitsma, Mick M. Welling, Ernest K. J. Pauwels, Henia Balter, Renata Mikolajczak, Patricia Oliver, Barbara Gorska
Rok vydání: 2005
Předmět:
Zdroj: Journal of Labelled Compounds and Radiopharmaceuticals. 48:683-691
ISSN: 1099-1344
0362-4803
DOI: 10.1002/jlcr.961
Popis: The purpose of this study was to investigate radiochemical and biological characteristics of an instant kit for the preparation of 99mTc-labelled UBI 29-41 for specific detection of infections. The kit is based on 99mTc-labelling via HYNIC conjugated to the terminal amine of the peptide, producing a well-understood labelled compound. One hour after the addition of fresh 99mTcO to the kit ITLC and HPLC reverse-phase analysis was performed. Stability of the labelled complex was challenged and the binding to bacterial pellets was assessed. Finally, the biodistribution and accumulation in MRSA-infected tissues were studied using scintigraphy and ex vivo countings. Data were compared to a non-kit control method. Radiochemical analysis indicated >96% labelling, stability for 24 h and the preparation was used without purification. In vitro studies showed 41% of radioactivity was bound to bacteria. After injection into mice with a bacterial infection the site of infection was visualized within 30 min. Kit prepared 99mTc-HYNIC-UBI 29-41 was rapidly (half-life 113 min) cleared via the kidneys and urinary bladder, essentially slower than control peptide (half-life 74 min). This slower clearance results in higher activities in blood and other tissues. Nevertheless, 99mTc-HYNIC-UBI 29-41 shows favourable radiochemical characteristics and deserves further evaluation in a clinical setting. Copyright © 2005 John Wiley & Sons, Ltd.
Databáze: OpenAIRE
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