Resistance to erythromycin of Staphylococcus spp. isolates from the food chain
| Autor: | H. Vlkova, M. Holasová, Jarmila Schlegelová, Vladimir Babak, Z. Jaglic, T. Stosova, P. Sauer |
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| Rok vydání: | 2008 |
| Předmět: |
0301 basic medicine
Lincosamides General Veterinary Streptogramin B biology medicine.drug_class 030106 microbiology Broth microdilution Erythromycin medicine.disease_cause biology.organism_classification Agar dilution Microbiology 03 medical and health sciences chemistry.chemical_compound chemistry Staphylococcus aureus Staphylococcus epidermidis medicine Staphylococcus medicine.drug |
| Zdroj: | Veterinární medicína. 53:307-314 |
| ISSN: | 1805-9392 0375-8427 |
| Popis: | The aim of this study was to determine both the occurrence and the genetic basis of resistance to erythromycin among 1 235 Staphylococcus spp. isolates obtained between 2000 and 2006 from (a) raw milk and meat (1 704 samples), (b) foodstuffs produced from these (451 samples), and (c) contact surfaces at processing plants and dairy farms (363 samples) in the Czech Republic. Isolates were screened by broth microdilution method for resistance to erythromycin and further 11 antimicrobial agents. In addition, isolates were screened by agar dilution (erythromycin range 1–128 mg/l) and D-zone test for inducible resistance to macrolides, lincosamides and streptogramin B (iMLSB). Forty isolates were found to be either resistant, or intermediate, to erythromycin (3.2% of isolates); of these, more than 50% were identified as S. epidermidis. A total of 15 (1.2%) resistant isolates of staphylococci originated from foodstuffs. Resistance mediated by methylation – i.e. iMLSB-resistance (10 isolates with the erm(A) or erm(C) gene) and constitutive MLSB-resistance (one isolate with the erm(B) and erm(C) genes) – exhibited a significantly high level of resistance to erythromycin with minimum inhibitory concentrations (MIC) of 64 – >128 mg/l (MICmode = >128 mg/l). In contrast, the efflux mechanism encoded by the msr(A) gene (13 isolates; MICrange = 4–128, MICmode = 128 mg/l), the inactivation mechanisms of resistance encoded by the mph(C) gene (three isolates; MICrange = 8–32 mg/l), and/or their combination (13 isolates; MICrange = 4–128, MICmode = 64 mg/l) led to lower MIC values. The efflux gene msr(A) dominated among the erythromycin-resistant isolates (65% of resistant isolates). This first report on the resistance of Staphylococcus spp. to erythromycin in the Czech Republic illustrates that, while occurrence was low, isolates from food were nevertheless carriers of erm(A), erm(B), erm(C), msr(A) and mph(C) genes for resistance to erythromycin and, therefore, represent a potential thread to humans. |
| Databáze: | OpenAIRE |
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