New Insights of Corynebacterium kroppenstedtii in Granulomatous Lobular Mastitis based on Nanopore Sequencing
| Autor: | Hong-Li Wu, Chuang Chen, Aisi Fu, Xin-Qian Li, Shengrong Sun, Jingping Yuan, Ran Liu, Tiangang Liu |
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| Rok vydání: | 2021 |
| Předmět: |
Bacilli
biology business.industry Corynebacterium Granulomatous mastitis biology.organism_classification medicine.disease law.invention Microbiology 03 medical and health sciences 0302 clinical medicine Gram staining Granulomatous lobular mastitis law 030220 oncology & carcinogenesis Medicine 030211 gastroenterology & hepatology Surgery Nanopore sequencing business human activities Bacteria Corynebacterium kroppenstedtii |
| Zdroj: | Journal of Investigative Surgery. 35:639-646 |
| ISSN: | 1521-0553 0894-1939 |
| DOI: | 10.1080/08941939.2021.1921082 |
| Popis: | BACKGROUND The etiology of granulomatous lobular mastitis (GLM) remains unknown. This study aimed to detect bacteria in GLM using Nanopore sequencing and identify the relationship between GLM and Corynebacterium kroppenstedtii. METHODS AND MATERIALS The bacterial detection on fresh samples (including breast pus and tissue) of 50 GLM patients using nanopore sequencing and culture methods. The bacterial detection rate of participants with different stages were compared and analyzed. Formalin-fixed and paraffin-embedded (FFPE) tissues from 39 patients were performed on Gram staining to identify Gram-positive bacilli (GPB) within lipid vacuoles. Moreover, the clinicopathological characteristics of GLM patients in different bacterial subgroups were also conducted. RESULTS In 50 GLM patients, the detection rate of bacteria was 78% using nanopore sequencing method, especially in the early stage of GLM (over 80%), which was significantly higher than that using culture methods (24%, p |
| Databáze: | OpenAIRE |
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