| Popis: |
The present study aimed to investigate comparative anti-inflammatory effects of Litsea japonica fruit and leaf extract considering the balance of safety and efficacy. Dose response studies were performed to determine the inhibitory effects of 70% EtOH extract of leaf (L70%) on the pro-inflammatory enzymes expression, COX-2/PGE2 and NO/iNOS, and pro-inflammatory cytokines production, IL-1β, IL-6, and TNF-α in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. We also examined comparative effects of 30 and 70% EtOH extract of fruits (F30% and F70%) at low concentration (10 ㎍/㎖) in the same conditions. L70% at 50 and 100 ㎍/㎖ showed inhibitory effects on almost all the inflammatory mediators we examined except for COX-2 regulation, but there were no effects at 10 ㎍/㎖. Since 100 ㎍/㎖ of L70% have 18.2% cytotoxicity, we compared the effects of fruit extract, F30% and F70% at 10 ㎍/㎖ on the regulation of NO/iNOS, PGE2, IL-1β, IL-6, and TNF-α and obtained that fruit extacts are more efficacious and safe than leaf. This study suggests that the 30% EtOH fraction of L. japonica fruit could be a good candidate for development as a functional food supplement in the prevention of inflammatory disorders. |
