Identification and characterization of a metal-resistant Acidithiobacillus ferrooxidans as important potential application for bioleaching
Autor: | Marziyeh Bahrami-Bavani, Yasaman Miyanmahaleh, Parisa Tajer-Mohammad-Ghazvini, Hassan Zare Tavakoli |
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Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
0301 basic medicine Plant Science Bacterial growth 01 natural sciences Biochemistry Ferrous Metal 03 medical and health sciences Bioremediation Bioleaching Genetics Yeast extract Food science Molecular Biology Ecology Evolution Behavior and Systematics biology Strain (chemistry) Chemistry Cell Biology biology.organism_classification 030104 developmental biology visual_art visual_art.visual_art_medium Animal Science and Zoology Bacteria 010606 plant biology & botany |
Zdroj: | Biologia. 76:1327-1337 |
ISSN: | 1336-9563 0006-3088 |
Popis: | Bioleaching is a common and eco-friendly method for the metals mobilization from mines, contaminated soils and wastes and it can be used in metal extraction and bioremediation. Due to the fact that these processes are considered as metabolism-dependent processes, isolation and identifying of the strains with high resistance features in responsible bacteria are one of the most important steps to have. In present study, an Iranian new isolate of iron-oxidizing bacteria was characterized. The phylogenetic analysis and 16S rRNA gene sequence of strain ZT-94 indicated that this strain is related to Acidithiobacillus ferrooxidans. It is investigated that the ferrous iron oxidation was increased dramatically in the early hours of bacterial growth and the ferrous iron (Fe2+) was completely oxidized at 30 h. According to the results, strain ZT-94 had the high tolerance capability to U, Ba, Al and Se. Iron oxidation by strain ZT-94 was inhibited by the addition of 312.5 mg/L Se and 70 mg/L Te but concentrations above 5000 mg/L of Ba and 2000 mg/L Al had no inhibitory effect on bacterial growth. In addition, strain ZT-94 was able to grow in the pH range of 1–4, temperatures at 25–35 °C. Results showed no inhibitory effects on bacterial growth rate in presence of 0.1 w/v organic compounds like fructose and glucose but a delayed growth was detected in presence of yeast extract in comparison with the others. The results showed that strain ZT-94 had high environmental adaptability and can be introduced as a suitable and valuable candidate for research in the bioleaching and bioremediation processes. |
Databáze: | OpenAIRE |
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