Antifungal activity of palmarosa (Cymbopogon martinii), tea tree (Melaleuca alternifolia) and star anise (Illicium verum) essential oils against Penicillium expansum and their mechanisms of action
| Autor: | Ludiana Canton, Marcelo Maraschin, Robson Marcelo Di Piero, Bruno Bachiega Navarro, Argus Cezar da Rocha Neto |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Antioxidant food.ingredient biology Chemistry medicine.medical_treatment Melaleuca alternifolia food and beverages 04 agricultural and veterinary sciences Antimicrobial biology.organism_classification 040401 food science 01 natural sciences Fungicide 0404 agricultural biotechnology food 010608 biotechnology medicine TBARS Food science Penicillium expansum Illicium verum Cymbopogon martinii Food Science |
| Zdroj: | LWT. 105:385-392 |
| ISSN: | 0023-6438 |
| DOI: | 10.1016/j.lwt.2019.02.060 |
| Popis: | Penicillium expansum is a cosmopolitan pathogen that can cause several damages to fruit crops, leading to an intense search for alternative methods for its control. This study aimed to assess the antimicrobial effects of Cymbopogon martinii, Illicium verum and Melaleuca alternifolia essential oils (EOs) against P. expansum, elucidating their mechanisms of action. The P. expansum conidia were exposed to the vapor phase of the EOs, in different concentrations (0.125 g L−1 to 1 g L−1), for different times (1–24 h). Their fungicidal/fungistatic effects assessed in vitro, and their mechanisms of action investigated through DNA, protein and glucose extravasation, lipid damage, reactive oxygen species accumulation and antioxidant activity. In situ, their antimicrobial activities were assessed on apples cv. Fuji. The EOs inhibited 100% of fungal germination after 4 h at 0.25 g L−1, causing damages to the plasma membrane, leading to DNA (∼69.27 ng), protein (∼39.28 μg) and glucose (∼5.77 mg) leakage, and lipid damage (∼7.72 pmol of TBARS). Although a high efficiency to inhibit P. expansum through an antioxidant activity was observed, a minor effect was observed in situ, indicating different interactions between EOs and the environment, which can reduce their antimicrobial activities. |
| Databáze: | OpenAIRE |
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