In vitro Medium TerConservation of Sweet Potato Genotypes Using Mannitol and Sorbitol

Autor: Mihaela Cioloca, Andreea Tican, Carmen Bădărău, Nina Bărăscu, Monica Popa
Rok vydání: 2021
Předmět:
Zdroj: Romanian Agricultural Research. 38:123-132
ISSN: 2067-5720
1222-4227
DOI: 10.59665/rar3813
Popis: The study is aimed at establishing an efficient protocol for ”in vitro” medium term conservation of sweet potato. Three medium variants and three different temperatures were used for this purpose. In this experiment 6 varieties of sweet potato were tested (Juhwangmi, KSC1, Yulmi, KSP1, Hayanmi and VSP1). Uninodal segments of fully developed sweet potato plants already established ”in vitro” were cultured on MS basal medium with and without osmotic regulators (mannitol and sorbitol). The cultures were incubated at three different temperatures: 24±2ºC, 20±2ºC and 16±2ºC. After 140 days of ”in vitro” conservation shoots height, number of leaves, number of roots and survival rate (%) were measured. Culture condition variants V1 (MS, 24±2ºC) and V2 (MS + 10g/l mannitol + 10 g/l sorbitol, 20±2ºC) ensured the best results regarding shoots height, number of leaves and number of roots. The highest survival rate (87.78% and 84.44%, respectively) were observed in the V2 and V3 (MS + 15g/l mannitol + 15 g/l sorbitol, 16±2ºC) culture condition variants. The slow growth technique allowed the successful ”in vitro” medium term conservation of sweet potato genotypes.
Databáze: OpenAIRE
Externí odkaz: