Validated TLC stability indicating methods for the quantitative determination of some veterinary drugs
| Autor: | Mohamed K. Abd El-Rahman, Mai A. Basha, Azza A. Moustafa, Lories I. Bebawy |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Florfenicol
Chromatography Silica gel 010401 analytical chemistry 02 engineering and technology 021001 nanoscience & nanotechnology Thiamphenicol 01 natural sciences Quantitative determination 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound chemistry Amprolium Stability indicating Forced degradation medicine Ethopabate 0210 nano-technology Spectroscopy medicine.drug |
| Zdroj: | Microchemical Journal. 146:157-163 |
| ISSN: | 0026-265X |
| DOI: | 10.1016/j.microc.2018.12.057 |
| Popis: | Sensitive and selective thin layer chromatographic methods have been developed for the analysis of three veterinary drugs. The first one is the determination of Amprolium HCL (AMP) and Ethopabate (ETHOP) in a mixture and in the presence of their degradation products. It was carried out on a TLC plates, silica gel 60 F 254 using acetonitrile-methanol-ammonium acetate buffer at pH 8.2 in the ratio 2:3:0.5 (v/v/v) as mobile phase. Quantification was carried out with UV detection at 274 nm and 272 nm for AMP and ETHOP, respectively. Forced degradation studies were performed using 4 M NaOH. The second one is the determination of Florfenicol (FLO) in presence of its related impurities Chlormphenicol (CLO) and Thiamphenicol (TIO). The analysis was carried out on a HPTLC plates, silica gel 60 F254 using chloroform-methanol-formic acid in the ratio 9:1:0.1 (v/v/v). Quantification was carried out with UV detection at 226 nm for FLO. Linearity ranges, mean recovery and relative standard deviation were calculated. The methods have been successfully applied for the determination of the cited drugs in bulk powder and in pharmaceutical formulations. The results obtained were statistically in full agreement when compared with a reported method. |
| Databáze: | OpenAIRE |
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