Biotype and macromolecular profiles of cytotoxin-producing strains ofHelicobacter pylorifrom antral gastric mucosa

Autor: Jane Bickley, María Remedios Foulquié Moreno, D. R. Morgan, Robert J. Owen, Menelaos Costas
Rok vydání: 1991
Předmět:
Zdroj: FEMS Microbiology Letters. 79:199-204
ISSN: 1574-6968
0378-1097
DOI: 10.1111/j.1574-6968.1991.tb04529.x
Popis: Biotype, genome, protein and plasmid profile diversitu amongst 40 epidemiologically unrelated strains of Helicobacter pylori was studied. Strains were API Zym biotypes II, III and IV but most (87%) were biotype II. Four subsets of strains were defined on a combination of motility (56% positive) and cytotoxin production (44% positive). A close association ( P = 0.45) between these two features was observed for 69% of strains. Each strain of H. pylori had a unique DNA type defined by either Hae III or Hind III total digest patterns and by ribopatterns, except for DNA of the rare strains not cut by these endonucleases. Strain diversity was confirmed by one-dimensional SDS-PAGE electrophoretic protein patterns. No consistent associations between cytotoxin activity and overall ribopattern or band subsets within a ribopattern were detected. Some strains (39%) contained a plasmid but the presence of plasmids was not consistently associated with either cytotoxin activity, biotype, motility or ribopattern. We conclude that the cytotoxin-producing strains of H. pylori were genomically as diverse as the non-cytotoxin producing strains.
Databáze: OpenAIRE
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