Vaccinia-specific CD4 T-cell responses in humans are predominantly restricted by HLA DR (132.22)
| Autor: | Lichen Jing, Stella Mayo McCaughey, D Huw Davies, Tiana M Chong, Phillip L Felgner, William W Kwok, Christopher B Wilson, David M Koelle |
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| Rok vydání: | 2009 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 182:132.22-132.22 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.182.supp.132.22 |
| Popis: | In humans, three to five loci encode cell-surface HLA class II heterodimers capable of binding peptides. Anecdotally, HLA DR dominates the restriction of pathogen-reactive CD4 T-cells. It is rare that primary cells have been examined on a per-cell basis, or that a large number of clones or unique fine specificities have been studied. Direct PBMC assays with multiple cytokine readouts show that HLA DR had the strongest contribution for vaccinia-specific memory CD4 T-cells. These data were reinforced by detailed investigation of the CD4 response in vaccinated persons. Amongst large panels of vaccinia-specific clones, most were DR-restricted. Genetic and proteomic tools were used to define a large number of CD4 specificities. A minimum of 42 novel CD4 antigenic regions were defined with peptides. The majority of evaluable specificities were HLA DR-restricted. This is the highest resolution data set available concerning the relative contributions of HLA class II loci to the CD4 response to a pathogen, and implies that antigen processing and presentation to HLA DR has a selective advantage in vivo in the context of live vaccinia infection. Supported by NIH AI40069 and AI067496 |
| Databáze: | OpenAIRE |
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