AAT Phenotype Identification by Isoelectric Focusing
| Autor: | Dina N. Greene, M. C. Elliott-Jelf, David G. Grenache |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Immunofixation
congenital hereditary and neonatal diseases and abnormalities biology Isoelectric focusing 030204 cardiovascular system & hematology Phenotype 03 medical and health sciences Electrophoresis chemistry.chemical_compound 0302 clinical medicine Isoelectric point Biochemistry chemistry 030220 oncology & carcinogenesis biology.protein Ph gradient Agarose Identification (biology) |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493971619 |
| DOI: | 10.1007/978-1-4939-7163-3_4 |
| Popis: | Isoelectric focusing (IEF) electrophoresis is considered to be the gold standard test for determining an individual's AAT phenotype. IEF electrophoresis is a technique used to separate proteins by differences in their isoelectric point (pI). Testing is performed on serum that is applied to an agarose gel containing ampholytes which create a pH gradient ranging from 4.2 to 4.9. Variants of AAT are therefore separated from each other and, after visualization of the focused protein bands using immunochemical techniques, can be identified and an AAT phenotype determined.In this chapter we elaborate on IEF electrophoresis as it relates to AAT phenotyping, describe practical approaches to AAT variant identification, and discuss circumstances in which phenotype testing may be inaccurate. |
| Databáze: | OpenAIRE |
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