Reconstruction of ROP GTPase Domains on the Plasma Membrane in Tobacco Leaves
| Autor: | Yoshihisa Oda, Hiroo Fukuda, Yoshinobu Nagashima |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology Effector Activator (genetics) Chemistry Nicotiana benthamiana GTPase biology.organism_classification 01 natural sciences Cell biology Cell wall 03 medical and health sciences 030104 developmental biology Membrane Microtubule Arabidopsis 010606 plant biology & botany |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493986118 |
| Popis: | Rho-type small GTPases (Rho GTPases) play central roles in various cellular events. Rho GTPases are often activated locally on the plasma membrane, forming plasma membrane domains, which induce downstream signaling. We describe an experimental procedure designed for inducing the production of de novo plasma membrane domains using Arabidopsis ROP11 GTPase. Introduction of ROP11 and its activator and inactivator into the tobacco leaf epidermis leads to formation of ROP11-activated plasma membrane domains on the plasma membrane. Effectors and marker genes can also be introduced alongside ROP11. This reconstruction system allows identifying molecules regulating Rho GTPase polarization. |
| Databáze: | OpenAIRE |
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