STUDIES ON THE PROPAGATION IN VITRO OF POLIOMYELITIS VIRUSES

Autor:	William F. Scherer, George O. Gey, Jerome T. Syverton
Rok vydání:	1953
Předmět:	biology Poliovirus Immunology medicine.disease_cause biology.organism_classification Virology Virus In vitro HeLa Epidermoid carcinoma Hemocytometer Cancer cell medicine biology.protein Immunology and Allergy Antibody
Zdroj:	Journal of Experimental Medicine. 97:695-710
ISSN:	1540-9538 0022-1007
DOI:	10.1084/jem.97.5.695
Popis:	The cells of a human epithelial cancer cultivated en masse have been shown to support the multiplication of all three types of poliomyelitis virus. These cells (strain HeLa of Gey) have been maintained in vitro since their derivation from an epidermoid carcinoma of the cervix in February, 1951. As the virus multiplied it caused in from 12 to 96 hours degeneration and destruction of the cancer cells. The specific destructive effect of the virus was prevented by adding homotypic antibody to the cultures but not by adding heterotypic antibodies. Methods for the preparation of large numbers of replicate cultures with suspensions of strain HeLa cells were described. The cells in suspension were readily quantitated by direct counts in a hemocytometer. A synthetic solution that maintains cellular viability was employed for viral propagation. The experimental results demonstrate the usefulness of strain HeLa cells for (a) the quantitation of poliomyelitis virus, (b) the measurement of poliomyelitis antibodies, and (c) the production of virus.
Databáze:	OpenAIRE
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