Two validated liquid chromatographic methods for the simultaneous determination of flumethasone pivalate, its related substance (flumethasone), and clioquinol
| Autor: | Mohammed Abdelkawy, Nour W. Sayed, Maha A. Hegazy, Eglal A. Abdelaleem, Rehab M. Abdelfatah |
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| Rok vydání: | 2014 |
| Předmět: |
Chromatography
Silica gel Clioquinol Clinical Biochemistry Analytical chemistry Biochemistry High-performance liquid chromatography Thin-layer chromatography Analytical Chemistry chemistry.chemical_compound Flumethasone pivalate chemistry Impurity Stationary phase medicine Flumethasone medicine.drug |
| Zdroj: | Journal of Planar Chromatography – Modern TLC. 27:466-471 |
| ISSN: | 1789-0993 0933-4173 |
| Popis: | Two liquid chromatographic methods were developed and validated. Simple and sensitive thin-layer chromatography (TLC)-densitometric and high-performance liquid chromatographic (HPLC) methods were used for the simultaneous determination of flumethasone pivalate (FP), flumethasone pivalate related substance and impurity, flumethasone (FL), and clioquinol (CL). The proposed TLC-densitometric method has been developed using silica gel plates 60 F254 as a stationary phase with benzene-hexane-acetone-formic acid (5:4:2:0.13, by volume) as a developing system followed by densitometric measurements at 235 nm. The studied components were quantified in the range of 0.3–4, 0.3–3, and 1.5–5 μg band−1, respectively. For HPLC method, chromatographic separation was achieved within 11 min with the required peak symmetry, accuracy, and precision on ODS column using acetonitrile-water (70:30, v/v) as the mobile phase at a flow rate of 1 mL min−1 with ultraviolet (UV) detection at 235 nm. The calibration plots were linear o... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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