PCR-based assay for the rapid detection of fumonisin-producingFusariumspecies in maize-based animal and poultry feeds in Karnataka, India

Autor:	Regina Sharmila Dass, G. R. Janardhana, Adkar Puroshothama Charith Raj, Marikunte Yanjarappa Sreenivasa
Rok vydání:	2009
Předmět:	Fusarium Veterinary medicine biology Bran business.industry food and beverages Contamination biology.organism_classification Rapid detection Husk law.invention Biotechnology chemistry.chemical_compound chemistry law Fumonisin Mycotoxin business Agronomy and Crop Science Polymerase chain reaction
Zdroj:	Archives Of Phytopathology And Plant Protection. 42:796-804
ISSN:	1477-2906 0323-5408
DOI:	10.1080/03235400701453295
Popis:	One hundred and fourteen animal feedstuffs and eighty poultry feed mixtures commonly used for animal and poultry nutrition in Karnataka, India, were analysed for Fusarium contamination. The total counts of fusaria in animal feeds and poultry feed mixtures revealed a high incidence of F. verticillioides, being isolated from all positive samples. Most contaminated samples were maize pellets (71.4%), cotton seed (66.6%), maize powder (60%) and fine wheat bran (50%), respectively, while no Fusarium species was isolated from Bengal gram husk and wheat flakes. All the Fusarium species were identified by the PCR method using genus specific ITS and group specific FUM 1 primers. Of the 374 Fusarium isolates tested with ITS set of primers, all fusaria scored positive, whereas only 244 (65%) isolates tested positive with the FUM 1 set of primers. The specificity of the primers provides the basis for a simple, accurate and precise detection of Fusarium species that represents fumonisin producers, which are a...
Databáze:	OpenAIRE
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