Evaluation of plasma-activated water (PAW) as a novel disinfectant: Effectiveness on Escherichia coli and Listeria innocua, physicochemical properties, and storage stability
| Autor: | Qingyang Wang, Deepti Salvi |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
biology Chemistry Disinfectant 04 agricultural and veterinary sciences biology.organism_classification medicine.disease_cause Antimicrobial 040401 food science 01 natural sciences Incubation period 0404 agricultural biotechnology 010608 biotechnology medicine Listeria Food science Escherichia coli Bacteria Food Science |
| Zdroj: | LWT. 149:111847 |
| ISSN: | 0023-6438 |
| DOI: | 10.1016/j.lwt.2021.111847 |
| Popis: | Plasma-activated water (PAW) is an emerging disinfectant with high antimicrobial activity due to a variety of reactive oxygen and nitrogen species. This study evaluated the inactivation efficacy of PAW against planktonic Escherichia coli and Listeria innocua as representatives of gram-negative and gram-positive bacteria, characterized the physicochemical properties of PAW, and investigated the storage stability of PAW. The effectiveness of PAW increased by extending the plasma activation time over water during PAW preparation or the incubation time of bacteria in PAW. PAW also showed higher effectiveness against E. coli than L. innocua: PAW5 (PAW prepared by plasma activation of water for 5 min) reduced E. coli by > 5 log CFU/mL and L. innocua by 1.8 ± 0.5 log CFU/mL within 5 min of bacterial incubation time, while PAW15 (PAW prepared by plasma activation of water for 15 min) reduced E. coli by > 5 log CFU/mL and L. innocua by 3.5 ± 0.6 log CFU/mL within 5 min of bacterial incubation time. Acidic pH and nitrogen species such as NO2− synergistically contributed to the inactivation efficacy of PAW. Fresh PAW showed the highest inactivation efficacy while refrigerated PAW maintained the inactivation efficacy compared to PAW stored at room temperature due to delayed dissipation of reactive species. |
| Databáze: | OpenAIRE |
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