Tissue dermal equivalent – а cellular product based on human dermal keratinocytes and fibroblasts: the properties of equivalent components and perspectives of practical application

Autor:	A. V. Butenka, Z. B. Kvacheva, I. B. Vasilevich, I. D. Volotovski, A. Ch. Chasnoit
Rok vydání:	2021
Předmět:	integumentary system Chemistry Product (mathematics) Materials Chemistry Cell biology Dermal equivalent
Zdroj:	Proceedings of the National Academy of Sciences of Belarus, Biological Series. 66:391-401
ISSN:	2524-230X 1029-8940
DOI:	10.29235/1029-8940-2021-66-4-391-401
Popis:	The nature and ways of isolation and cultivation in vitro of keratinocytes and fibroblasts, the main cellular components of skin to prepare a new biomedical product, tissue dermal equivalent were considered. The main attention was payed to optimization of upbuilding dermal cell biomass including selection of medium compositions and conditions of cultivation. The information was given on main parameters of cell cultures as proliferation activity, viability and phenotype of the cells. Genotoxicity of fibroblasts and biocompatibility of the cells with organic matrixes to find the optimal carrier for cellular elements of tissue dermal equivalent were studied. The composition, the process of preparation of tissue dermal equivalent and perspectives of its practical application were discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::eb8ac6a6d82b2e53004d3f7cf5dd0ab6 https://doi.org/10.29235/1029-8940-2021-66-4-391-401 Zobrazit plný text záznamu