Monoclonal antibodies detecting different epitopes on the Forssman glycolipid hapten
| Autor: | A Sonnenberg, P van Balen, T Hengeveld, G J Kolvenbag, R P Van Hoeven, J Hilgers |
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| Rok vydání: | 1986 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 137:1264-1269 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.137.4.1264 |
| Popis: | Two hybridomas, derived by fusing mouse myeloma cells with spleen cells from a rat immunized with mouse mammary tumors, have been shown to produce antibodies that recognize cell surface antigens on mesenchymal cells in a variety of tissues. Evidence presented in this report suggests that these antibodies detect overlapping epitopes on the Forssman glycolipid hapten (GalNAc alpha 1-3GalNAc beta 1-3Gal alpha 1-4Gal beta 1-4Glc beta 1-1Cer). One antibody (33B12) reacts with the terminal sugar sequence GalNAc alpha 1-3GalNAc and is specific for Forssman. The other antibody (117C9) recognizes the internal sugar sequence GalNAc beta 1-3Gal. The terminal sugar sequence GalNAc beta 1-3Gal in globoside, as well as the internal sugar sequence GalNAc beta 1-4Gal in asialo-GM1, is not recognized as an antigenic determinant by 117C9. Nevertheless, the 117C9 antibody does not react exclusively with the Forssman antigen. In a lipid extract fractionated by Folch partition of mouse mammary tumors, the antibody also detects other glycolipids. |
| Databáze: | OpenAIRE |
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