On Penicillin-Binding Protein 1b Affinity-Labeling Reagents
| Autor: | Lothar Hennig, Mohammed Daghish, Sabine Giesa, Matthias Findeisen, Daniela Volke, Peter Welzel, Ramona Oehme |
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| Rok vydání: | 2003 |
| Předmět: |
chemistry.chemical_classification
Trifluoromethyl Affinity labeling Chemistry Organic Chemistry Photoaffinity Labels Conjugated system Ligand (biochemistry) Biochemistry Combinatorial chemistry Catalysis Inorganic Chemistry chemistry.chemical_compound Enzyme Biotin Drug Discovery Physical and Theoretical Chemistry Binding site |
| Zdroj: | Helvetica Chimica Acta. 86:4214-4232 |
| ISSN: | 1522-2675 0018-019X |
| DOI: | 10.1002/hlca.200390346 |
| Popis: | The synthesis of some 3-aryl-3-(trifluoromethyl)3H-diazirine and benzophenone-based photoaffinity labels is reported. The photolabile group is bound to a scaffold that also accommodates functional groups to which an indicator unit (biotin) and the bioactive ligand can be attached orthogonally. To three of the labels, moenomycin was conjugated with the aim to provide tools for the identification of the moenomycin binding site within the transglycosylase domain of the enzyme PBP 1b. Some preliminary photoaffinity-labeling experiments were carried out. |
| Databáze: | OpenAIRE |
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