Downregulation ofHotairor LSD1 Impaired Heart Regeneration in the Neonatal Mouse

Autor:	Bing Yang, Shuying Wang, Manman Qiu, Guanwei Fan, Ling Zhang, Sipei Zhang, Bo Zhang, Qiaoman Fei, Qin Wang
Rok vydání:	2021
Předmět:	Gene knockdown animal structures biology Regeneration (biology) HOTAIR Cell Biology General Medicine Cell biology Downregulation and upregulation RNA interference Genetics biology.protein Demethylase Molecular Biology Gene HOX Transcript Antisense RNA
Zdroj:	DNA and Cell Biology. 40:1177-1184
ISSN:	1557-7430 1044-5498
DOI:	10.1089/dna.2021.0095
Popis:	Previous studies have shown that lysine-specific demethylase 1 (LSD1) could regulate cell cycle progression through demethylation. The 3'domain of HOX transcript antisense RNA (Hotair) combined with the LSD1/CoREST/REST complex helps LSD1 target the corresponding gene. However, its role in mice's myocardial regeneration is still unclear. The heart from neonatal mice shows strong myocardial regeneration ability, but this ability disappears 7 days after birth. Our study shows that the myocardial tissue highly expresses Hotair and Lsd1 within 1 week after birth, consistent with the myocardial regeneration time window. Knockdown Lsd1 or Hotair expression by RNA interference could inhibit myocardial regeneration and cardiomyocyte proliferation. Our results suggest that Hotair-mediated demethylation of LSD1 may play an important role in myocardial regeneration in neonatal mice.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::eb841328378aebf4bd05b4b94500d9e4 https://doi.org/10.1089/dna.2021.0095 Zobrazit plný text záznamu