Novel optical approaches for label-free quantification of nano-cytotoxic effects
| Autor: | Sarah Mues, Steffi Ketelhut, Jürgen Schnekenburger, Jan Antunovic, Björn Kemper |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.diagnostic_test Chemistry Cell Nanoparticle Nanotechnology 01 natural sciences Flow cytometry Nanomaterials 010309 optics 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Nanotoxicology 0103 physical sciences medicine Biophysics Viability assay Cytotoxicity Intracellular |
| Zdroj: | SPIE Proceedings. |
| ISSN: | 0277-786X |
| Popis: | Commonly used cytotoxicity assays to determine the formation of reactive oxygen species, cell viability or cell death are often affected by applied nanomaterials, which lead to false-positive or false-negative results. Thus, novel nanomaterial toxicity testing strategies that allow for high nanomaterial doses to determine Low Effect Levels (LOEL) even of low toxic materials are of high interest. We demonstrate novel approaches to quantify cytotoxic effects with new parameter sets such as cellular refractive index, volume, density and dry mass that are obtained by digital holographic microscopy (DHM). Furthermore, we correlate results obtained from spherical (NM 300) and rod shaped (NM 302) silver nanomaterials with established cell viability and cell death assays. Moreover, in a label-free flow cytometry configuration, cell-nanoparticle-interaction-kinetics were determined by side scatter signal analysis. We demonstrate that silver spheres show a higher cytotoxicity than silver rods and found that this effect correlates with a decrease of the intracellular refractive index and a decreased temporal development of dry mass and cell covered surface area indicating reduced cell viability and increased cell death. Results from side scatter analysis suggest a dose-dependent uptake kinetics of both materials that correlates with cytotoxicity data of the established assays. Taken together, our results demonstrate DHM and flow cytometry as promising novel label-free tools for nanomaterial toxicity and cell particle interaction studies. |
| Databáze: | OpenAIRE |
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