122 Successful Burkholderia spp. eradication with hypothiocyanite/lactoferrin. In vitro study evidence over a worldwide collection of clinical strains
| Autor: | A. Payet-Burin, V. Juarez-Perez, Y. Sonmez, C. Bechetoille, S. Perrotto |
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| Rok vydání: | 2015 |
| Předmět: |
Pulmonary and Respiratory Medicine
biology Inoculation Lactoferrin Negative control Hypothiocyanite Antimicrobial biology.organism_classification Microbiology chemistry.chemical_compound Burkholderia cepacia complex Burkholderia chemistry Pediatrics Perinatology and Child Health biology.protein In vitro study |
| Zdroj: | Journal of Cystic Fibrosis. 14:S88 |
| ISSN: | 1569-1993 |
| DOI: | 10.1016/s1569-1993(15)30299-x |
| Popis: | Objectives To test the efficacy of various combinations of OSCN − and bLF on clinical Burkholderia strains. Methods We tested 165 Burkholderia spp. CF-clinical isolates from worldwide origin. MIC values were obtained according to CLSI guidelines with minor modifications and Time-kill curves with standard methods. All experiments were done in triplicate. Results 100% of isolates tested were inhibited by OSCN − alone within a range of 30–157 mg/mL. In contrast, only 4.8% (8/165) of the tested strains are inhibited by bLF alone within a range of 0.25–96 mg/mL. The FIC analysis of the whole panel of strains reveals that inhibitory combinations are predominantly additive or synergistic for 73% of the collection. The combination of 118 mg/mL of OSCN − with 8 mg/mL of bLF inhibits 100% of tested isolates. Time-kill curves performed on 21 strains. Results show that the OSCN − /bLF combination (ALX-009) decreases the bacterial count by 2–4 logs at 6 h after inoculation when compared to the inoculum and by 4–6 logs if compared to the negative control. 24 h after inoculation, the bacterial counts were reduced by 4–6 logs if compared to the negative control. These results suggest that for time 0–6 h after inoculation OSCN − exerts an important antimicrobial activity that is then maintained by bLF over time. Conclusion The data obtained by Alaxia on 165 clinical isolates of Burkholderia cepacia complex and other Burkholderia spp. demonstrates the high bactericidal potential of the OSCN − /bLF combination (ALX-009) and reinforces both the previous published data and the interest to developing this combination to respond to the unmet medical need of Burkholderia spp. infections in CF patients. |
| Databáze: | OpenAIRE |
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