Cloning and Expression of Fasciola gigantica Cathepsin-B Recombinant Proteins

Autor:	Savita Bisen, M. Silamparasan, Rohit Lall, O.K. Raina, Andleeb Aftab, S.C. Yadav
Rok vydání:	2020
Předmět:	Cathepsin Proteases General Veterinary biology Fasciola gigantica medicine.disease biology.organism_classification Cathepsin B law.invention Biochemistry Antigen Affinity chromatography law Recombinant DNA medicine Animal Science and Zoology Fasciolosis
Zdroj:	Indian Journal of Animal Research.
ISSN:	0976-0555 0367-6722
DOI:	10.18805/ijar.b-3959
Popis:	Fasciola gigantica cathepsin (cysteine) proteases are potential diagnostic antigens for animal and human fasciolosis. These include cathepsin-L proteases that have been exploited in the diagnosis of animal fasciolosis. However, no scientific data on the diagnostic potential of F. gigantica cathepsin B proteases is available. Therefore, three recombinant antigens of F. gigantica viz. cathepsin (cat) B-1, cat B-2 and cat B-3 were expressed in prokaryotic expression system. The recombinant antigens were purified under denaturing conditions by Nickel affinity chromatography and an optimal level of the recombinant proteins was obtained. These recombinant proteins will further be evaluated for their potential in the early prepatent diagnosis of F. gigantica infection in domestic ruminants.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::eb64cffab96508d94d3cdcfa47bc6903 https://doi.org/10.18805/ijar.b-3959 Zobrazit plný text záznamu