| Autor: |
Semik, E., Ząbek, T., Gurgul, A., Fornal, A., Szmatoła, T., Pawlina, K., Wnuk, M., Klukowska-Rötzler, Jolanta, Koch, Christoph, Mählmann, K., Bugno-Poniewierska, M. |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
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| DOI: |
10.7892/boris.108413 |
| Popis: |
OBJECTIVE: In this study, for the first time we report the genome-wide DNA methylation profile of skin tumour in horses and describe differentially methylated genomic regions (DMRs) with respect to healthy skin. MATERIALS & METHODS: The comparative analysis of DNA methylation patterns detected using Reduced Representation Bisulfite Sequencing (RRBS) technique, allowed identification of 136 regions showing differential methylation between sarcoid and normal skin tissue. RESULTS: Most of the identified DMRs were short fragments, less than 1 kb in size, located in the intergenic regions. Among identified DMRs there were also regions located within genes directly or indirectly related with oncogenesis. We additionally validated 9 CpG sites showing hypomethylation and 9 CpG sites that were hypermethylated in lesional sample, confirming the identified changes in the DNA methylation. CONCLUSION: Knowledge on the changes taking place in the process of DNA methylation may provide a basis for the development of new alternative diagnostic or therapeutic approaches to equine sarcoids. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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