| Popis: |
T HE MICRO-'TITRATION technique for serologic tests was first described in the Hungarian literature by Takat,sy and associates (1). Sever (2) modified the technique and applied it to complement fixation, hemagglutination, hemagglutination-inhibition, and metabolic-inhibition tests. Rosenbaum and coworkers (3) further applied the technique to the serum neutralization test.. The,ir results indica.ted that serum neutralization by micro technique reduced time, material, and effort and agreed well with resu.lts obtained by conventional tissue-culture tube methods. Our application of this method to the determination of neutralizing antibody titers, in paired serums, in a group of children who had received attenuated type I poliovirus vaccine produced results similar to those of Rosenbaum and associates (3). We also applied this micro method to the identification of polioviruses isolated from another group of children who had received trivalent attenuated poliovirus vaccine. |
