Layer-layer assembly for extremely stable glucose sensors

Autor:	Golam Faruque Khan
Rok vydání:	1997
Předmět:	food.ingredient Fabrication Materials science biology Analytical chemistry Gelatin Amperometry Analytical Chemistry chemistry.chemical_compound food chemistry Electrode Electrochemistry biology.protein Glucose oxidase Glutaraldehyde Polarization (electrochemistry) Layer (electronics)
Zdroj:	Electroanalysis. 9:533-536
ISSN:	1521-4109 1040-0397
DOI:	10.1002/elan.1140090705
Popis:	A novel fabrication of an amperometric glucose sensor by layer after layer approach is described. The sensor electrode is fabricated by arranging a layer of Pt black, a layer of glucose oxidase (GOD) and a layer of stabilizer gelatin on a shapable electro-conductive (SEC) film surface. Finally, the dried layered-assembly is cross-linked by exposing to a diluted glutaraldehyde solution. The performance of the developed sensor is evaluated by a FIA system at 37°C and under a continuous polarization at 0.4 V (vs. Ag/AgCl). The sensitivity of the sensor was dependent on the amount of GOD loaded. The highest sensitivity (3.6 μA/mM cm−2) of the sensor was obtained at a GOD loading of 160 μg/cm2, and the linear dynamic range was extended to 80 mM level when the sensor was covered with a polycarbonate membrane. The sensor shows an extremely stable response for several weeks and a storage stability of over 2 years.
Databáze:	OpenAIRE
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