Reduction of linoleic acid inhibition in production of conjugated linoleic acid by Propionibacterium freudenreichii ssp. shermanii
| Autor: | Marjatta Vahvaselkä, Tarja Suomalainen, Auli Rainio, Simo Laakso |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
biology
Chemistry Propionibacterium freudenreichii Linoleic acid Conjugated linoleic acid Immunology food and beverages Sorbitan Monooleate Growth inhibitory General Medicine biology.organism_classification Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Linoleic acid metabolism Biochemistry Genetics Food science Molecular Biology Whey permeate |
| Zdroj: | Canadian Journal of Microbiology. 47:735-740 |
| ISSN: | 1480-3275 0008-4166 |
| Popis: | A method for the production of conjugated linoleic acid (CLA) from linoleic acid (LA) using growing cultures of Propionibacterium freudenreichii ssp. shermanii JS was developed. The growth inhibitory effect of LA was eliminated by dispersing it in a sufficient concentration of polyoxyethylene sorbitan monooleate detergent. For the whey permeate medium used, the optimum LA:detergent ratio was 1:15 (w/w). As a result, the cultures tolerated at least 1000 microg x mL(-1) LA, which was converted to CLA with 57%-87% efficiency. The cis-9, trans-11 and trans-9, cis-11 isomers constituted 85%-90% of the CLA produced. The feasibility of the method was demonstrated also in de Man Rogosa-Sharpe (MRS) broth. |
| Databáze: | OpenAIRE |
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