Microfabricated Centrifugal Microfluidic Systems: Characterization and Multiple Enzymatic Assays
| Autor: | Gregory J. Kellogg, Joe Lin, and Norman F. Sheppard, David C. Duffy, Heather L. Gillis |
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| Rok vydání: | 1999 |
| Předmět: | |
| Zdroj: | Analytical Chemistry. 71:4669-4678 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/ac990682c |
| Popis: | This paper describes a microfluidic system in which fluids are pumped by centrifugal force through microscopic channels defined in a plastic disk in order to perform complex analytical processes. The channels are created either by casting poly(dimethylsiloxane) against molds fabricated by photolithography or by conventional machining of poly(methyl methyacrylate). The channels have a wide range of diameters (5 μm−0.5 mm) and depths (16 μm−3 mm). Fluids are loaded into reservoirs near the center of the disk, the disk is rotated on the shaft of a simple motor at 60−3000 rpm, and the fluids are pumped outward by centrifugal force through microfluidic networks. The control of flow in the time domain, i.e., gating, is achieved by the use of passive valves based on capillary forces. Flow rates ranging from 5 nL/s to >0.1 mL/s have been achieved using channels of different dimensions and different rates of rotation. The method of pumping is insensitive to many physicochemical properties of the liquid, such as pH... |
| Databáze: | OpenAIRE |
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