Production of novel fusarielins by ectopic activation of the polyketide synthase 9 cluster in Fusarium graminearum
| Autor: | Reinhard Wimmer, Teis Esben Sondergaard, T. Verne Lee, Frederik Teilfeldt Hansen, Stig Purup, Jens Laurids Sørensen, Henriette Giese, Louise Graabæk Klitgaard, Dan Staerk, Rasmus John Normand Frandsen |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Fusarium
biology Metabolite Mutant Secondary metabolite biology.organism_classification Microbiology chemistry.chemical_compound chemistry Biochemistry Polyketide synthase Botany biology.protein medicine Expression cassette Transcription factor Gene Ecology Evolution Behavior and Systematics medicine.drug |
| Zdroj: | Environmental Microbiology. 14:1159-1170 |
| ISSN: | 1462-2912 |
| DOI: | 10.1111/j.1462-2920.2011.02696.x |
| Popis: | Summary Like many other filamentous fungi, Fusarium graminearum has the genetic potential to produce a vast array of unknown secondary metabolites. A promising approach to determine the nature of these is to activate silent secondary metabolite gene clusters through constitutive expression of cluster specific transcription factors. We have developed a system in which an expression cassette containing the transcription factor from the targeted PKS cluster disrupts the production of the red mycelium pigment aurofusarin. This aids with identification of mutants as they appear as white colonies and metabolite analyses where aurofusarin and its intermediates are normally among the most abundant compounds. The system was used for constitutive expression of the local transcription factor from the PKS9 cluster (renamed FSL) leading to production of three novel fusarielins, F, G and H. This group of compounds has not previously been reported from F. graminearum or linked to a biosynthetic gene in any fungal species. The toxicity of the three novel fusarielins was examined against colorectal cancer cell lines where fusarielin H was more potent than fusarielin F and G. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text