Function analysis and stress-mediated cis-element identification in the promoter region of VqMYB15
Autor: | Dong Duan, Ruixiang Li, Fanding Zhu |
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Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine Functional analysis Abiotic stress Promoter Plant Science Biology 01 natural sciences Cell biology Function analysis 03 medical and health sciences 030104 developmental biology Vitis quinquangularis Binding site Transcription factor Function (biology) 010606 plant biology & botany |
Zdroj: | Plant Signaling & Behavior. 15:1773664 |
ISSN: | 1559-2324 |
DOI: | 10.1080/15592324.2020.1773664 |
Popis: | The transcription factor MYB15 plays an important role in grape basal immunity, and its promoter can be used as a potential target in resistance breeding. However, the regulatory mechanisms of cis-elements in its promoter region under a variety of stresses remain unclear. In this study, we identified some putative cis-regulatory elements present upstream of MYB15 in Vitis quinquangularis Shanyang (pVqMYB15_SY) and subsequently characterized the function of these elements using reporter assays. Our results showed that TCA-elements 1 and 2, ABRE, MYC and 3-AF1 binding site 1 are key cis-regulatory elements in pVqMYB15_SY and play important roles in plant bio/abiotic stress resistance. |
Databáze: | OpenAIRE |
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