Cloning and function analysis of a type 2 diacylglycerol acyltransferase (DGAT2) from Perilla frutescens
| Autor: | Tao Zhang, Dan Li, Jun-Xing Lu, Tian Hu, Xin Tang, Qiu-Yun Hu, Geng Lu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Linolenic acid Linoleic acid food and beverages Fatty acid Plant Science Palmitic acid chemistry.chemical_compound Oleic acid chemistry lipids (amino acids peptides and proteins) Arachidonic acid Stearic acid Food science Agronomy and Crop Science Unsaturated fatty acid Biotechnology |
| Zdroj: | Acta Agronomica Sinica. 46:1283-1290 |
| ISSN: | 0496-3490 |
| DOI: | 10.3724/sp.j.1006.2020.94192 |
| Popis: | Diacylglycerol acyltransferase (DGAT) is a key enzyme in the final step of triacylglycerol (TAG) synthesis in plant. In seed oil of certain plants, DGAT2 can selectively accumulate more unsaturated fatty acids. In this paper, we successful cloned PfDGAT2 from Perilla frutescens and performed bioinformatics analysis. Real-time fluorescence quantitative analysis showed that PfDGAT2 was expressed in different organs, with the highest in seeds at 10 d after anthesis, the medium in roots, and gradual decrement in the middle and late stages of seed. Compared with the wild-type, the oil content of seeds in PfDGAT2 transgenic Arabidopsis was increased by 21.68%–77.89%. The fatty acid components of the four strains with the largest increase in seed oil content were analyzed. Compared with the control, linolenic acid (C18:3), arachidonic acid (C20:1), arachidonic acid (C20:2), docosaenoic acid (C22:1) increased significantly by 4.57%, 7.44%, 5.40%, and 0.37%, respectively. Palmitic acid (C16:0), stearic acid (C18:0), and linoleic acid (C18:2) were obviously reduced by 3.47%, 6.64%, and 4.83%, respectively. Oleic acid (C18:1) only decreased by 0.18% and arachidic acid (C20:0) by 1.91%. In conclusion that PfDGAT2 gene can not only increase the oil content, but promote the accumulation of unsaturated fatty acids such as linolenic acid and arachidonic acid, which provides a reference and theoretical basis for studying the synthesis and accumulation of unsaturated fatty acid in plants. |
| Databáze: | OpenAIRE |
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