| Autor: |
A.G. Brownell, Arthur Lietze, Robert J. DeLange, J.J. Chang, Marshall R. Urist, Y. K. Huo |
| Rok vydání: |
1987 |
| Předmět: |
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| Zdroj: |
Peptide Growth Factors-Part A ISBN: 9780121820466 |
| Popis: |
Publisher Summary Among the cellular and extracellular components of the organic matrix of dentin, bone, and various tumors is a bone morphogenetic protein (BMP). The identity of BMP is based upon its capacity to induce the differentiation of mesenchymal-type perivascular connective tissue cells into the bone. This chapter discusses a standard method of preparing BMP from either bovine or human bone. There are also some methods of cleavage of the molecular structure of BMP to produce the smallest unit of protein structure with osteoinductive activity, designated as bone morphogenetic polypeptide (BMP-p). The most available source of BMP is demineralized bovine or human cortical bone matrix. The most direct method for the preparation of BMP consists of the chemical extraction of HCl-demineralized bone in a 4 M solution of GuHCl. This extract consists of a complex mixture of intra- and extracellular proteins that is extremely difficult to fractionate. A slightly more manageable extract with a high level of BMP activity is obtainable from insoluble gelatinized bone matrix prepared by sequentially extracting the relatively soluble noncollagenous proteins. This removes about 20% of the non-BMP relatively soluble proteins from the system. The BMP is tightly bound to insoluble protein aggregates within the densely packed cross-linked structure of cortical bone matrix. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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