Elimination of Fibrinogen Interference in a Dye-Binding Method for Iron

Autor:	S L Perkins, N E Tokessy, Daylily S. Ooi
Rok vydání:	1992
Předmět:	Chromatography medicine.diagnostic_test Chemistry Dye binding Biochemistry (medical) Clinical Biochemistry Heparin Interference (genetic) Serum samples Fibrinogen Coulometry Reagent medicine Serum iron medicine.drug
Zdroj:	Clinical Chemistry. 38:541-544
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/38.4.541
Popis:	Serum iron concentrations greater than 90 mumol/L were measured in samples from hemodialysis patients by a Ferrozine dye-binding method. Reanalysis by coulometry showed these results to be spuriously high. Turbidity resulting from precipitation of fibrinogen was identified as the source of the interference. High concentrations of heparin enhanced the effect. We conclude that the persistence of fibrinogen in serum samples from patients treated with anticoagulants is a potential interference in Ferrozine dye-binding methods performed without prior deproteinization. Adding guanidine.HCl to the acid buffer reagent is a simple way to eliminate this interference.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::eafea2cb0df7d411c3adf0a3d6dd4f88 https://doi.org/10.1093/clinchem/38.4.541 Zobrazit plný text záznamu