Site-Specific 32P-Labeling of Cytokines, Monoclonal Antibodies, and Other Protein Substrates for Quantitative Assays and Therapeutic Application

Autor:	William A. Clark, Doranelly Philipova, Lei Lin, Wei Wu, Lara S. Izotova, Sidney Pestka
Rok vydání:	2002
Předmět:	Chemistry Kinase medicine.drug_class Monoclonal antibody Molecular biology General Biochemistry Genetics and Molecular Biology Membrane protein Biochemistry Consensus sequence medicine Phosphorylation Target protein Protein kinase A Peptide sequence Biotechnology
Zdroj:	BioTechniques. 33:S76-S87
ISSN:	1940-9818 0736-6205
DOI:	10.2144/oct0209
Popis:	Radiolabeled proteins are used in a variety of laboratory applications as well as in radioimmunotherapy. This review focuses on methods that utilize genetic engineering to introduce exogenous phosphorylation sites into proteins. Protein kinase substrate sites can be introduced into target proteins to serve as tags for several purposes. Because many protein kinases, each preferring a unique consensus sequence, are well characterized, the essential structure and function of the target protein can be effectively preserved through judicious selection and design of the phosphate incorporation site. After phosphorylation, these proteins are often indistinguishable from the parent molecules in assays of functional or biological activity. This convenient approach permits incorporation of 32P, 33P, 35S, or nonradioactive 31P, and is rapid, efficient, and safe. Most importantly, 32P labeling of monoclonal antibodies or other therapeutic protein candidates has several significant advantages over radioiodination or chemical conjugation of heavy metal isotopes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::eaf92d6131caa092275049761b9f1b4e https://doi.org/10.2144/oct0209 Zobrazit plný text záznamu