ICP-MS-assisted identification of selenium-containing proteins in 2D gels using a new capillary HPLC–ICP MS interface and Orbitrap tandem mass spectrometry
| Autor: | Juliusz Bianga, Joanna Szpunar |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Chromatography
Laser ablation Chemistry Capillary action Analytical chemistry chemistry.chemical_element Tandem mass spectrometry Orbitrap Mass spectrometry Analytical Chemistry law.invention chemistry.chemical_compound law Acetonitrile Inductively coupled plasma mass spectrometry Spectroscopy Selenium |
| Zdroj: | J. Anal. At. Spectrom.. 28:288-292 |
| ISSN: | 1364-5544 0267-9477 |
| DOI: | 10.1039/c2ja30273j |
| Popis: | An improved instrumental approach is discussed for the identification of Se-containing proteins separated by 2D polyacrylamide gel electrophoresis. A protein, detected in a gel by laser ablation ICP-MS 2D 78Se imaging, was excised from another gel obtained in parallel under identical conditions, and digested with trypsin. Selenium-peptides were detected by capillary HPLC–ICP MS using a new robust commercial interface and an ICP mass spectrometer fitted with a frequency-matching RF generator facilitating work at high concentrations of acetonitrile in the mobile phase. The identification of selenopeptides was carried out by means of hybrid linear quadrupole trap-Orbital trap MS offering a higher intrascan dynamic range and better mass accuracy than the hitherto used Q-TOF (time-of-flight) mass spectrometers. An example of the identification of Se-containing glyceraldehyde-3-phosphate dehydrogenase-3 in yeast is discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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