Red blood cell associated IgG in normal and pathologic states
| Autor: | I.O. Szymanski, Paul R. Odgren, LM Snyder, NL Fortier |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Hemolytic anemia
medicine.medical_specialty Immunology Spherocytosis Population Biochemistry Immunoglobulin G Hereditary spherocytosis Coombs test Internal medicine medicine education education.field_of_study biology medicine.diagnostic_test hemic and immune systems Cell Biology Hematology medicine.disease Red blood cell Agglutination (biology) medicine.anatomical_structure Endocrinology biology.protein circulatory and respiratory physiology |
| Zdroj: | Blood. 55:48-54 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v55.1.48.48 |
| Popis: | We studied the anti-IgG-induced agglutination of both normal and abnormal red blood cells (RBC) using a sensitive, automated antiglobulin test. Normal RBC agglutinated strongly with anti-IgG antibody, indicating that IgG was present on the erythrocyte membrane. Young RBC, recovered by centrifugation from a normal RBC population, agglutinated with anti-IgG less than the old cells, suggesting that immunoglobulin G accumulated gradually on the RBC membrane in vivo. The degree of anti-IgG-induced RBC agglutination correlated negatively with the reticulocyte count and positively with the concentration of plasma IgG. RBC from patients with hypogammaglobulinemia appeared to have a low subnormal quantity of membrane-bound IgG, whereas the reverse was the case in hypergammaglobulinemia. During hemolytic episodes, RBC of patients with hereditary spherocytosis agglutinated poorly with anti- IgG, apparently due to predominance of young RBC. RBC of patients with nonspherocytic. Coombs-negative, nonimmune hemolytic anemia usually also agglutinated poorly with anti-IgG. However, in some cases of active hemolytic anemia, decreased agglutination with anti-IgG was not observed, suggesting that these young RBC had increased amounts of membrane-bound IgG. |
| Databáze: | OpenAIRE |
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