Direct immunofluorescence and enzyme-linked immunosorbent assays for evaluating organic contaminant degrading bacteria

Autor:	M. M. Franck, J.S Bray, Carl B. Fliermans, K.D Lanclos, R.L Brigmon, D.F Scott
Rok vydání:	1998
Předmět:	Microbiology (medical) biology medicine.diagnostic_test Methanotroph Chemistry Biodegradation biology.organism_classification Immunofluorescence Microbiology Bioremediation Microbial population biology Polyclonal antibodies medicine biology.protein Antibody Molecular Biology Bacteria
Zdroj:	Journal of Microbiological Methods. 32:1-10
ISSN:	0167-7012
DOI:	10.1016/s0167-7012(97)00092-4
Popis:	Immunological procedures were developed to enumerate chlorinated hydrocarbon (CH) degrading bacteria in environmental samples. Polyclonal antibodies (Pabs) were produced by immunizing New Zealand white rabbits against 18 CH-degrading bacteria. These bacteria included methanotrophic and chlorobenzene (CB)-degrading species. An enzyme-linked immunosorbent assay (ELISA) was used to test for cross-reactivity and sensitivity of the Pabs. Direct fluorescent antibodies (DFAs) were developed against selected methanotrophic bacteria isolated from a trichloroethylene (TCE)-contaminated landfill at the Savannah River Site (SRS) and cultures from the American Type Culture Collection (ATCC). Analysis of ELISA results showed some of the Pabs to be group specific while others were species specific. The threshold of sensitivity for the ELISA is 105 bacteria cells/ml. Results from the DFA and ELISA techniques for enumeration of methanotrophic bacteria in groundwater were higher but not significantly different (P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::eacd0aeeb185bf1331b3fa7a1622d6f1 https://doi.org/10.1016/s0167-7012(97)00092-4 Zobrazit plný text záznamu Full Text from ScienceDirect