Frankiaproduces a hydroxamate siderophore under iron limitation

Autor:	Dallas B. Aronson, Gregory L. Boyer
Rok vydání:	1992
Předmět:	Siderophore Growth cycle Catechol biology Strain (chemistry) Physiology Frankia biology.organism_classification Ceanothus americanus Microbiology chemistry.chemical_compound Hplc assay chemistry Actinorhizal plant Agronomy and Crop Science
Zdroj:	Journal of Plant Nutrition. 15:2193-2201
ISSN:	1532-4087 0190-4167
DOI:	10.1080/01904169209364468
Popis:	Frankia strain 52065, a nitrogen‐fixing symbiont of the actinorhizal plant, Ceanothus americanus, was grown under both iron‐replete and iron‐limited culture conditions. Cells were monitored over time for both growth and siderophore production. Culture media was tested for the presence of siderophores using a combination of chemical and Chromatographic techniques. Both iron‐replete and iron‐limited cultures tested negative for catechol structures using the Arnow assay. The Csaky test for hydroxamate structures showed an increase in activity with increasing age of culture, reaching a maximal production of 87 nmolar N per ml supernatant in iron‐limited cultures of Frankia on day 65. Iron‐replete cultures were consistently negative for the presence of hydroxamates throughout their growth cycle. An HPLC assay for the detection of iron‐55 labelled siderophores indicated a single iron‐binding compound eluting at 12.3 minutes. This peak was first detected in iron‐limited culture supernatant during the ea...
Databáze:	OpenAIRE
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