Impact of Glyphosate on the Rhizosphere Microbial Communities of An EPSPS-Transgenic Soybean Line ZUTS31 by Metagenome Sequencing
| Autor: | Jing Cheng, Xiao-Mei Hua, Lei Zhang, Jin-Liang Qi, Yong-Hua Yang, Xiao-Ming Wang, Yan-Jun Pang, Rong-Wu Yang, Gu-Hao Wang, Yin-Ling Zhu, Gui-Hua Lu, Huixia Shou |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Rhizosphere Beta diversity food and beverages 010501 environmental sciences Biology 16S ribosomal RNA biology.organism_classification 01 natural sciences 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Microbial population biology Agronomy Metagenomics Glyphosate Botany Genetics Nitrogen fixation Genetics (clinical) Bacteria 0105 earth and related environmental sciences |
| Zdroj: | Current Genomics. 19 |
| ISSN: | 1389-2029 |
| DOI: | 10.2174/1389202918666170705162405 |
| Popis: | Background The worldwide use of glyphosate has dramatically increased, but also has been raising concern over its impact on mineral nutrition, plant pathogen, and soil microbiota. To date, the bulk of previous studies still have shown different results on the effect of glyphosate application on soil rhizosphere microbial communities. Objective This study aimed to clarify whether glyphosate has impact on nitrogen-fixation, pathogen or disease suppression, and rhizosphere microbial community of a soybean EPSPS-transgenic line ZUTS31 in one growth season. Method Comparative analysis of the soil rhizosphere microbial communities was performed by 16S rRNA gene amplicons sequencing and shotgun metagenome sequencing analysis between the soybean line ZUTS31 foliar sprayed with diluted glyphosate solution and those sprayed with water only in seed-filling stage. Results There were no significant differences of alpha diversity but with small and insignificant difference of beta diversity of soybean rhizosphere bacteria after glyphosate treatment. The significantly enriched Gene Ontology (GO) terms were cellular, metabolic, and single-organism of biological process together with binding, catalytic activity of molecular function. The hits and gene abundances of some functional genes being involved in Plant Growth-Promoting Traits (PGPT), especially most of nitrogen fixation genes, significantly decreased in the rhizosphere after glyphosate treatment. Conclusion Our present study indicated that the formulation of glyphosate-isopropylamine salt did not significantly affect the alpha and beta diversity of the rhizobacterial community of the soybean line ZUTS31, whereas it significantly influenced some functional genes involved in PGPT in the rhizosphere during the single growth season. |
| Databáze: | OpenAIRE |
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