Two putative zinc metalloproteases contribute to the virulence of Clostridium perfringens strains that cause avian necrotic enteritis

Autor:	Anthony L. Keyburn, Mark E Ford, Robert J. Moore, Volker Haring, Ben Wade, Julian I. Rood
Rok vydání:	2020
Předmět:	Whole genome sequencing 0303 health sciences Metalloproteinase Proteases General Veterinary 030306 microbiology Mutant Virulence Clostridium perfringens Biology medicine.disease_cause Microbiology Pathogenesis 03 medical and health sciences medicine Gene 030304 developmental biology
Zdroj:	Journal of Veterinary Diagnostic Investigation. 32:259-267
ISSN:	1943-4936 1040-6387
DOI:	10.1177/1040638719898689
Popis:	Two putative zinc metalloproteases encoded by Clostridium perfringens have been implicated in the pathogenesis of necrotic enteritis, an economically significant poultry disease that is caused by this anaerobic bacterium. These proteases have ~64% amino acid identity and are encoded by the zmpA and zmpB genes. We screened 83 C. perfringens isolates by PCR for the presence of these genes. The first gene, zmpB, is chromosomally located and was present in all screened strains of C. perfringens, regardless of their origin and virulence. The second gene, zmpA, is plasmid-borne and was only found in isolates derived from chickens with necrotic enteritis. We describe the generation of insertionally inactivated mutants of both zmpA and zmpB in a virulent C. perfringens isolate. For each mutant, a significant ( p
Databáze:	OpenAIRE
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