Production of milk-derived bioactive peptides as precursor chimeric proteins in chloroplasts of Chlamydomonas reinhardtii

Autor: Luis Martínez-Salgado, Luz María Teresita Paz-Maldonado, Nohemí Campos-Quevedo, Juan Carlos Guevara-Arauza, Ruth Elena Soria-Guerra, Sergio Rosales-Mendoza
Rok vydání: 2012
Předmět:
Zdroj: Plant Cell, Tissue and Organ Culture (PCTOC). 113:217-225
ISSN: 1573-5044
0167-6857
DOI: 10.1007/s11240-012-0261-3
Popis: Bioactive peptides are considered high value-added ingredients in functional foods, and the main sources of these are milk, egg, plants, among others. A major limitation in their commercial use is the cost of production. This study deals with the design and production of a chimeric protein in chloroplasts of the microalgae Chlamydomonas reinhardtii to generate bioactive peptides of antihypertensive, opioid, antimicrobial, and hypocholesterolemic activities. A synthetic gene, designated as NCQ, coding for the selected chimeric protein, is transferred to C. reinhardtii using biolistic bombardment. Transplastomic transformants have been identified by PCR and Western blots following selection on a spectinomycin-containing medium. An ELISA quantification assay has revealed that the expressed NCQ protein accumulated at levels ranging between 0.16 and 2.4 % of total soluble protein. These findings demonstrate that chloroplasts of C. reinhardtii could serve as a robust expression platform for production of bioactive peptides.
Databáze: OpenAIRE
Externí odkaz: